Louis Winthorpe's (Dan Aykroyd) house – 2014 Delancey Place, Philadelphia, Pennsylvania.
The Duke & Duke bank – Wells Fargo Building, 135 S. Broad Street, Philadelphia, Pennsylvania
The Dukes mansion – Mill Neck Manor, 40 Frost Mill Road, Mill Neck, New York.
The Heritage Club – Curtis Institute of Music, 1726 Locust Street, Philadelphia, Pennsylvania.
Billy Ray Valentine (Eddie Murphy) pretends to be a blind cripple – Rittenhouse Square, S. 18th Street at Locust Street, Philadelphia, Pennsylvania.
The police station – Community College of Philadelphia, 1700 Spring Garden Street, Philadelphia, Pennsylvania.
The bar where Billy Ray is showing off – Cannon's Pub, 2794 Broadway, New York, New York. Many thanks to David Wright for pointing that out. Cannon's had closed in 2004 after 70 years in business and returned a few months later as O'Connell's. The pub was remodeled in 2013 and reopened as Tara Hill Irish Tavern but closed again in July 2016.
Ophelia (Jamie Lee Curtis) agrees to help Louis – Centre Square, 1500 Market Street, Philadelphia, Pennsylvania. The Clothespin sculpture is a 45-foot work of public art created by artist Claes Oldenburg in 1975. It was installed for the Bicentennial Celebration in 1976, and has remained there ever since.
Louis sees Billy Ray passing by in a limousine – Independence Hall, 520 Chestnut Street, Philadelphia, Pennsylvania.
Ophelia's apartment – 1210 South Street, Philadelphia, Pennsylvania. The neighborhood has since changed, and the street is barely recognizable.
The depressed Louis gets off the bus – Avenue B between E. 14th and 13th Streets, New York, New York. Many thanks to AlphaJ for identifying this location.
The Dukes' henchman Clarence Beeks (Paul Gleason) calling from 'Washington, DC' – Centre Street near Foley Square, New York, New York
The train leaving Washington, DC – Newark Penn Station, 1048 Raymond Boulevard, Newark, New Jersey.
Arriving in Philadelphia – 30th Street Station, 3001 Market Street, Philadelphia, Pennsylvania.
Louis and Billy Ray outsmart the Dukes on the trading floor – World Trade Center, New York, New York. The original World Trade Center was destroyed in the September 11 terrorist attacks in 2001. The site was rebuilt with five new skyscrapers and a memorial to the victims of the attacks. One World Trade Center opened in October 2014. It is the tallest building in the U.S., and already a recognizable New York landmark.
Beeks and a gorilla are loaded onto a ship heading for Africa – Brooklyn Bridge Park Pier 2, New York, New York.
Louis, Billy Ray, Ophelia and Coleman vacation on a tropical beach – Davis Bay, St. Croix, U.S. Virgin Islands
A Fish Called Wanda
The Million Pound Note